qpcR (version 1.0-6)

rutledge: qPCR dilution experiment with replicates and different runs from Rutledge et al (Biorad Opticon)

Description

A dilution experiment of a 102 bp amplicon with six 10-fold dilutions, with four replicates each in five independent runs.

Usage

data(rutledge)

format

A data frame with the PCR cycles and and the six 10-fold dilutions, with four replicates each and five independent runs, organized as X.Ry.Z, with x = the dilution number, y = the run number and z = the replicate number.

source

Supplemental data 1 to the paper.

Details

The real-time PCR was conducted for a 102 bp amplicon in an Opticon2 instrument.

References

Sigmoidal curve-fitting redefines quantitative real-time PCR with the prospective of developing automated high-throughput applications. Rutledge RG, Nucleic Acids Research, 2004, 32, e178.

Examples

data(rutledge)
### on single data
m1 <- multdrc(X1.R1.1 ~ Cycles, data = rutledge, fct = l3())
pcrplot(m1)
### on replicated data
repData <- repform(rutledge[,1:5], c(0,1,1,1,1))
m2 <- multdrc(values ~ Cycles, curve = Curve, data = repData, fct = l5())
pcrplot(m2, type = "all")