qpcR (version 1.2-2)

eff: The amplification efficiency curve of a fitted object

Description

Calculates the efficiency curve from the fitted object by $E = \frac{F(n)}{F(n-1)}$, with E = efficiency, F = raw fluorescence, n = Cycle number.

Usage

eff(object, sequence = NULL, plot = FALSE)

Arguments

object

an object of class 'pcrfit'.

sequence

a 3-element vector (from; to; by) defining the sequence for the efficiency curve, defaults to [min(Cycles), max(Cycles)] with 100 points per cycle.

plot

should the efficiency be plotted?

Value

• A list with the following components:
• eff.x
  the cycle points.
• eff.y
  the efficiency values at eff.x.
• effmax.x
  the cycle number with the highest efficiency.
• effmax.y
  the maximum efficiency.

Examples

m <- pcrfit(reps, 1, 2, l5)

## with default 100 points per cycle
eff(m, plot = TRUE) 

## not all data and only 10 points per cycle
eff(m, sequence = c(5, 35, 0.1), plot = TRUE)