qpcR (version 1.2-2)

guescini1: A qPCR dilution experiment with replicates (Lightcycler 480) from Guescini et al

Description

A high quality 10-fold dilution experiment with 7 dilution steps and 12 replicates each.

Usage

data(guescini1)

format

A data frame with the PCR cycles and 84 qPCR runs with 12 replicates of seven 10-fold dilutions. The replicates are defined by FX.Y (X = dilution number, Y = replicate number).

source

Supplemental data 1 to the paper.

Details

The real-time PCR was conducted with primers for the NADH dehydrogenase 1 in a Lightcycler 480 (Roche). The data is background subtracted.

References

A new real-time PCR method to overcome significant quantitative inaccuracy due to slight amplification inhibition. Guescini M et al, BMC Bioinformatics, 2008, 9: 326.

Examples

data(guescini1)
ml <- modlist(guescini1, model = l4)
plot(ml)