qpcR (version 1.2-4)

S27: PCR data from 15 different testicular biopsies (Roche Lightcycler)

Description

The data was obtained from cDNA reverse transcribed from the isolated RNA of 15 different testicular biopsies.

Usage

data(S27)

format

A data frame with cycle number and 15 different qPCR runs (F1 - F15).

source

Andrej-Nikolai Spiess & Caroline Feig, Department of Andrology, University Hospital Hamburg, Germany.

Details

The real-time PCR was conducted with primers for the S27a housekeeping gene in a Lightcycler 1.0 instrument (Roche Diagnostics).

Examples

data(S27)
m1 <- pcrfit(S27, 1, 2, l5)
plot(m1)