TcTS

<p>The variable 'a' is the concentration of p-nitrophenyl alpha-sialoside (as donor) in mM.
The variable 'b' is the lactose (as acceptor) concentration in mM.
'rate' is given in mM/min.
Biochemistry 2008, 47, 3507–3512 (https://pubmed.ncbi.nlm.nih.gov/18284211)</p>

datasets

Contains utilities for the analysis of Michaelian kinetic data. Beside the classical linearization
methods (Lineweaver-Burk, Eadie-Hofstee, Hanes-Woolf and Eisenthal-Cornish-Bowden), features include the
ability to carry out weighted regression analysis that, in most cases, substantially improves the estimation
of kinetic parameters (Aledo (2021) <doi:10.1002/bmb.21522>). To avoid data transformation and the potential
biases introduced by them, the package also offers functions to directly fitting data to the Michaelis-Menten
equation, either using ([S], v) or (time, [S]) data. Utilities to simulate substrate progress-curves (making
use of the Lambert W function) are also provided. The package is accompanied of vignettes that aim to orientate
the user in the choice of the most suitable method to estimate the kinetic parameter of an Michaelian enzyme.

Juan Aledo

renz

R-Enzymology

TcTS function

<p>A dataframe with 16 rows (conditions assayed) and 3 columns (variables).</p>

TcTS: Kinetic data for Trypanosoma cruzi trans-sialidase.

Description

Usage

Arguments

Format