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rsahmi (version 0.0.2)

taxa_counts: Quantitation of microbes

Description

After identifying true taxa, reads assigned to those taxa are extracted and then undergo a series of filters. The cell barcode and UMI are used to demultiplex the reads and create a barcode x taxa counts matrix. The full taxonomic classification of all resulting barcodes and the number of counts assigned to each clade are tabulated.

Usage

taxa_counts(umi_list, samples = NULL)

Value

A polars DataFrame.

Arguments

umi_list

A list of polars DataFrame for UMI data returned by prep_dataset.

samples

A character of sample identifier for each element in umi_list.

See Also

Examples

Run this code
if (FALSE) {
# `umi_list` should be the output of all samples from `prep_dataset()`, and 
# filtered by `slsd()` and `blsd()`
taxa_counts(umi_list, basename(names(umi_list)))
}

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