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rtpcr (version 2.1.1)

qPCR Data Analysis

Description

Tools for qPCR data analysis using Delta Ct and Delta Delta Ct methods, including t-tests, ANOVA, ANCOVA, repeated-measures models, and publication-ready visualizations. The package supports multiple target, and multiple reference genes, and uses a calculation framework adopted from Ganger et al. (2017) and Taylor et al. (2019) , covering both the Livak and Pfaffl methods.

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Install

install.packages('rtpcr')

Monthly Downloads

520

Version

2.1.1

License

GPL-3

Issues

1

Pull Requests

0

Stars

1

Forks

1

Maintainer

Ghader Mirzaghaderi

Last Published

January 8th, 2026

Functions in rtpcr (2.1.1)

ANOVA_DDCt

Delta Delta Ct ANOVA analysis
ANOVA_DCt

Delta Ct ANOVA analysis
REPEATED_DDCt

Delta Delta Ct ANOVA analysis on repeated measure data
TTEST_DDCt

Delta Delta Ct method t-test analysis
data_3factor

Sample data (three factor)
data_1factor_one_ref_Eff

Sample qPCR data (two different conditions)
data_2factorBlock

Sample data in (two factor with blocking factor)
data_efficiency

Sample qPCR data: amplification efficiency
data_2factorBlock3ref

Sample data in (two factor with blocking factor and 3 reference genes)
multiplot

Combine multiple ggplot objects into a single layout
efficiency

Amplification efficiency statistics and standard curves
plotFactor

Bar plot of gene expression for 1-, 2-, or 3-factor experiments
meanTech

Calculate mean of technical replicates
Means_DDCt

Delta Ct or Delta Delta Ct pairwise comparisons using a fitted model
data_repeated_measure_1

Repeated measure sample data
data_1factor_one_ref

Sample qPCR data (two different conditions)
data_repeated_measure_2

Repeated measure sample data
globalVariables

Internal global variables
data_withTechRep

Sample data (with technical replicates)
data_1factor

Sample data (one factor three levels)
data_1factor_Two_ref

Sample qPCR data (one target and two reference genes under two different conditions)
data_2factor

Sample data (two factor)
Lee_etal2020qPCR

Sample data (with technical replicates)
data_2factor3ref

Sample data in (two factor with blocking factor)