data_Yuan2006PMCBioinf

A sample qPCR data set with one experimental factor (condition)
and one reference gene. Each line belongs to a separate individual
(non-repeated measure experiment).

internal

Tools for qPCR data analysis using Delta Ct and Delta Delta Ct methods, including t-tests, ANOVA, ANCOVA, repeated-measures models, and publication-ready visualizations. The package supports multiple target, and multiple reference genes, and uses a calculation framework adopted from Ganger et al. (2017) <doi:10.1186/s12859-017-1949-5> and Taylor et al. (2019) <doi:10.1016/j.tibtech.2018.12.002>, covering both the Livak and Pfaffl methods.

Ghader Mirzaghaderi

rtpcr

qPCR Data Analysis

data_Yuan2006PMCBioinf function

A data frame with 24 observations and 6 variables:<dl>
 <dt>condition</dt>
<dd>Experimental factor with two levels (control, treatment)</dd> <dt>rep</dt>
<dd>Biological replicates</dd> <dt>target</dt>
<dd>Mean amplification efficiency of target gene</dd> <dt>Ct_target</dt>
<dd>Ct values of target gene. Each is the mean of technical replicates</dd> <dt>ref</dt>
<dd>Mean amplification efficiency of reference gene</dd> <dt>Ct_ref</dt>
<dd>Ct values of reference gene. Each is the mean of technical replicates</dd>
</dl>

Format

Sample data in (one factor with one reference gene) — data_Yuan2006PMCBioinf

A data frame with 24 observations and 6 variables:<dl>
 <dt>condition</dt>
<dd>Experimental factor with two levels (control, treatment)</dd>

 <dt>rep</dt>
<dd>Biological replicates</dd>

 <dt>target</dt>
<dd>Mean amplification efficiency of target gene</dd>

 <dt>Ct_target</dt>
<dd>Ct values of target gene. Each is the mean of technical replicates</dd>

 <dt>ref</dt>
<dd>Mean amplification efficiency of reference gene</dd>

 <dt>Ct_ref</dt>
<dd>Ct values of reference gene. Each is the mean of technical replicates</dd>


</dl>

data_Yuan2006PMCBioinf: Sample data in (one factor with one reference gene)

Description

Usage

Arguments

Format