data_repeated_measure_2

A repeated measure sample data in which 6 individuals have been analysed. In the "id" column, a unique number is assigned to each individual, e.g. all the three number 1 indicate one individual.
samples are taken or measurements are scored over different time points (time column) from each individual.

internal

Tools for qPCR data analysis using Delta Ct and Delta Delta Ct methods, including t-tests, ANOVA, ANCOVA, repeated-measures models, and publication-ready visualizations. The package supports multiple target, and multiple reference genes, and uses a calculation framework adopted from Ganger et al. (2017) <doi:10.1186/s12859-017-1949-5> and Taylor et al. (2019) <doi:10.1016/j.tibtech.2018.12.002>, covering both the Livak and Pfaffl methods.

Ghader Mirzaghaderi

rtpcr

qPCR Data Analysis

data_repeated_measure_2 function

A data frame with 18 observations and 7 variables:<dl>
 <dt>treatment</dt>
<dd>treatment</dd> <dt>time</dt>
<dd>time course levels</dd> <dt>id</dt>
<dd>experimental factor</dd> <dt>Target</dt>
<dd>Amplification efficiency of target gene</dd> <dt>Target_Ct</dt>
<dd>Ct of target gene</dd> <dt>Ref</dt>
<dd>Amplification efficiency of reference gene</dd> <dt>Ref_Ct</dt>
<dd>Ct of reference gene</dd>
</dl>

Format

Repeated measure sample data — data_repeated_measure_2

A data frame with 18 observations and 7 variables:<dl>
 <dt>treatment</dt>
<dd>treatment</dd>

 <dt>time</dt>
<dd>time course levels</dd>

 <dt>id</dt>
<dd>experimental factor</dd>

 <dt>Target</dt>
<dd>Amplification efficiency of target gene</dd>

 <dt>Target_Ct</dt>
<dd>Ct of target gene</dd>

 <dt>Ref</dt>
<dd>Amplification efficiency of reference gene</dd>

 <dt>Ref_Ct</dt>
<dd>Ct of reference gene</dd>


</dl>

data_repeated_measure_2: Repeated measure sample data

Description

Usage

Arguments

Format