data_withTechRep

A sample data for calculating biological replicated. Each line belongs to a separate individual (non-repeated measure experiment).

internal

Tools for qPCR data analysis using Delta Ct and Delta Delta Ct methods, including t-tests, ANOVA, ANCOVA, repeated-measures models, and publication-ready visualizations. The package supports multiple target, and multiple reference genes, and uses a calculation framework adopted from Ganger et al. (2017) <doi:10.1186/s12859-017-1949-5> and Taylor et al. (2019) <doi:10.1016/j.tibtech.2018.12.002>, covering both the Livak and Pfaffl methods.

Ghader Mirzaghaderi

rtpcr

qPCR Data Analysis

data_withTechRep function

A data frame with 18 observations and 9 variables:<dl>
 <dt>Condition</dt>
<dd>experimental factor</dd> <dt>biolrep</dt>
<dd>biological replicate</dd> <dt>techrep</dt>
<dd>technical replicates</dd> <dt>target</dt>
<dd>Amplification efficiency of target gene</dd> <dt>Ct_target</dt>
<dd>Ct of target gene</dd> <dt>ref</dt>
<dd>Amplification efficiency of reference gene</dd> <dt>Ct_ref</dt>
<dd>Ct of reference gene</dd>
</dl>

Format

Sample data (with technical replicates) — data_withTechRep

A data frame with 18 observations and 9 variables:<dl>
 <dt>Condition</dt>
<dd>experimental factor</dd>

 <dt>biolrep</dt>
<dd>biological replicate</dd>

 <dt>techrep</dt>
<dd>technical replicates</dd>

 <dt>target</dt>
<dd>Amplification efficiency of target gene</dd>

 <dt>Ct_target</dt>
<dd>Ct of target gene</dd>

 <dt>ref</dt>
<dd>Amplification efficiency of reference gene</dd>

 <dt>Ct_ref</dt>
<dd>Ct of reference gene</dd>


</dl>

data_withTechRep: Sample data (with technical replicates)

Description

Usage

Arguments

Format