data_Lee_etal2020qPCR

A sample data for calculating biological replicated. Each line belongs to a separate individual (non-repeated measure experiment).

internal

Tools for qPCR data analysis using Delta Ct and Delta Delta Ct methods, including t-test, wilcox.test, ANOVA models, and publication-ready visualizations. The package supports multiple target, and multiple reference genes, and uses a calculation framework adopted from Ganger et al. (2017) <doi:10.1186/s12859-017-1949-5> and Taylor et al. (2019) <doi:10.1016/j.tibtech.2018.12.002>, covering both the Livak and Pfaffl methods.

Ghader Mirzaghaderi

rtpcr

qPCR Data Analysis

data_Lee_etal2020qPCR function

A data frame with 72 observations and 8 variables:<dl>
 <dt>factor1</dt>
<dd>experimental factor</dd> <dt>DS</dt>
<dd>DS</dd> <dt>biolRep</dt>
<dd>biological replicate</dd> <dt>techRep</dt>
<dd>technical replicates</dd> <dt>APOE</dt>
<dd>Amplification efficiency of APOE gene</dd> <dt>Ct_APOE</dt>
<dd>Ct of APOE gene</dd> <dt>GAPDH</dt>
<dd>Amplification efficiency of GAPDH gene</dd> <dt>Ct_GAPDH</dt>
<dd>Ct of GAPDH gene</dd>
</dl>

Format

Sample data (with technical replicates) — data_Lee_etal2020qPCR

A data frame with 72 observations and 8 variables:<dl>
 <dt>factor1</dt>
<dd>experimental factor</dd>

 <dt>DS</dt>
<dd>DS</dd>

 <dt>biolRep</dt>
<dd>biological replicate</dd>

 <dt>techRep</dt>
<dd>technical replicates</dd>

 <dt>APOE</dt>
<dd>Amplification efficiency of APOE gene</dd>

 <dt>Ct_APOE</dt>
<dd>Ct of APOE gene</dd>

 <dt>GAPDH</dt>
<dd>Amplification efficiency of GAPDH gene</dd>

 <dt>Ct_GAPDH</dt>
<dd>Ct of GAPDH gene</dd>


</dl>

data_Lee_etal2020qPCR: Sample data (with technical replicates)

Description

Usage

Arguments

Format