data_efficiency1

A sample qPCR dataset for demonstrating efficiency calculation.

internal

Tools for qPCR data analysis using Delta Ct and Delta Delta Ct methods, including t-test, wilcox.test, ANOVA models, and publication-ready visualizations. The package supports multiple target, and multiple reference genes, and uses a calculation framework adopted from Ganger et al. (2017) <doi:10.1186/s12859-017-1949-5> and Taylor et al. (2019) <doi:10.1016/j.tibtech.2018.12.002>, covering both the Livak and Pfaffl methods.

Ghader Mirzaghaderi

rtpcr

qPCR Data Analysis

data_efficiency1 function

A data frame with 21 observations and 4 variables:<dl>
 <dt>Dilutions</dt>
<dd>Dilution factor</dd> <dt>C2H2.26</dt>
<dd>Target gene 1</dd> <dt>C2H2.01</dt>
<dd>Target gene 2</dd> <dt>GAPDH</dt>
<dd>Reference gene</dd>
</dl>

Format

Sample qPCR data: amplification efficiency 1 — data_efficiency1

A data frame with 21 observations and 4 variables:<dl>
 <dt>Dilutions</dt>
<dd>Dilution factor</dd>

 <dt>C2H2.26</dt>
<dd>Target gene 1</dd>

 <dt>C2H2.01</dt>
<dd>Target gene 2</dd>

 <dt>GAPDH</dt>
<dd>Reference gene</dd>


</dl>

data_efficiency1: Sample qPCR data: amplification efficiency 1

Description

Usage

Arguments

Format