data_efficiency_Yuan2006PMCBioinf

A sample qPCR dataset for demonstrating efficiency calculation.

internal

Tools for qPCR data analysis using Delta Ct and Delta Delta Ct methods, including t-test, wilcox.test, ANOVA models, and publication-ready visualizations. The package supports multiple target, and multiple reference genes, and uses a calculation framework adopted from Ganger et al. (2017) <doi:10.1186/s12859-017-1949-5> and Taylor et al. (2019) <doi:10.1016/j.tibtech.2018.12.002>, covering both the Livak and Pfaffl methods.

Ghader Mirzaghaderi

rtpcr

qPCR Data Analysis

data_efficiency_Yuan2006PMCBioinf function

A data frame with 12 observations and 5 variables:<dl>
 <dt>dilutions</dt>
<dd>dilution factor</dd> <dt>ref_control</dt>
<dd>reference gene in control condition</dd> <dt>ref_treatment</dt>
<dd>reference gene in treatment condition</dd> <dt>target_control</dt>
<dd>target gene in control condition</dd> <dt>target_treatment</dt>
<dd>Reference gene in treatment condition</dd>
</dl>

Format

Sample qPCR data: amplification efficiency 2 — data_efficiency_Yuan2006PMCBioinf

A data frame with 12 observations and 5 variables:<dl>
 <dt>dilutions</dt>
<dd>dilution factor</dd>

 <dt>ref_control</dt>
<dd>reference gene in control condition</dd>

 <dt>ref_treatment</dt>
<dd>reference gene in treatment condition</dd>

 <dt>target_control</dt>
<dd>target gene in control condition</dd>

 <dt>target_treatment</dt>
<dd>Reference gene in treatment condition</dd>


</dl>

data_efficiency_Yuan2006PMCBioinf: Sample qPCR data: amplification efficiency 2

Description

Usage

Arguments

Format