hclust_haystack_highD

Function for hierarchical clustering of genes according to their distribution in a higher-dimensional space.

One key exploratory analysis step in single-cell genomics data analysis
is the prediction of features with different activity levels. For example, we want
to predict differentially expressed genes (DEGs) in single-cell RNA-seq data,
spatial DEGs in spatial transcriptomics data, or differentially accessible
regions (DARs) in single-cell ATAC-seq data. 'singleCellHaystack' predicts differentially
active features in single cell omics datasets without relying on the clustering
of cells into arbitrary clusters. 'singleCellHaystack' uses Kullback-Leibler
divergence to find features (e.g., genes, genomic regions, etc) that are active
in subsets of cells that are non-randomly positioned inside an input space (such as
1D trajectories, 2D tissue sections, multi-dimensional embeddings, etc). For
the theoretical background of 'singleCellHaystack' we refer to our original paper
Vandenbon and Diez (Nature Communications, 2020) <doi:10.1038/s41467-020-17900-3>
and our update Vandenbon and Diez (Scientific Reports, 2023) <doi:10.1038/s41598-023-38965-2>.

Alexis Vandenbon

singleCellHaystack

A Universal Differential Expression Prediction Tool for
Single-Cell and Spatial Genomics Data

Diego Diez

hclust_haystack_highD function

<dl><dt>x</dt>
<dd>Coordinates of cells in a 2D or higher-dimensional space. Rows represent cells, columns the dimensions of the space.</dd>
<dt>detection</dt>
<dd>A logical matrix showing which genes (rows) are detected in which cells (columns)</dd>
<dt>genes</dt>
<dd>A set of genes (of the 'detection' data) which will be clustered.</dd>
<dt>method</dt>
<dd>The method to use for hierarchical clustering. See '?hclust' for more information. Default: "ward.D".</dd>
<dt>grid.coordinates</dt>
<dd>Coordinates of grid points in the same space as 'x', to be used to estimate densities for clustering.</dd>
<dt>scale</dt>
<dd>whether to scale data.</dd></dl>

Arguments

Function for hierarchical clustering of genes according to their distribution in a higher-dimensional space. — hclust_haystack_highD

<dl>

<dt>x</dt>
<dd>Coordinates of cells in a 2D or higher-dimensional space. Rows represent cells, columns the dimensions of the space.</dd>


<dt>detection</dt>
<dd>A logical matrix showing which genes (rows) are detected in which cells (columns)</dd>


<dt>genes</dt>
<dd>A set of genes (of the 'detection' data) which will be clustered.</dd>


<dt>method</dt>
<dd>The method to use for hierarchical clustering. See '?hclust' for more information. Default: "ward.D".</dd>


<dt>grid.coordinates</dt>
<dd>Coordinates of grid points in the same space as 'x', to be used to estimate densities for clustering.</dd>


<dt>scale</dt>
<dd>whether to scale data.</dd>

</dl>

hclust_haystack_highD: Function for hierarchical clustering of genes according to their distribution in a higher-dimensional space.

Description

Usage

Value

Arguments

Examples