estimate

Estimate the initial kinetic parameters, <code>kOn0</code> and <code>kOff0</code>, and save in the returned "Bli" object. These parameters can serve as the starting value in the next step, <code>fitKinetics</code>, to calculate the kinetics binding model of the small-molecule under investigation.

 manip 

Bio-Layer Interferometry (BLI) is a technology to determine the binding kinetics between biomolecules. BLI signals are small and noisy when small molecules are investigated as ligands (analytes). We develop this package to process and analyze the BLI data acquired on Octet Red96 from Fortebio more accurately.
Sun Q., Li X., et al (2020) <doi:10.1038/s41467-019-14238-3>.
In this new version, we organize the BLI experiment data and analysis methods into a S4 class with self-explaining structure.

Qingan Sun

smoke

Small Molecule Octet/BLI Kinetics Experiment

estimate function

<dl> <dt>obj</dt>
<dd><code>obj</code> of "Bli" class</dd></dl>

Arguments

tools:::Rd_package_author("smoke")
Maintainer: tools:::Rd_package_maintainer("smoke")

Author

Estimate initial kinetic parameters — estimate

<dl>

 <dt>obj</dt>
<dd><code>obj</code> of "Bli" class</dd>

</dl>

estimate: Estimate initial kinetic parameters

Description

Usage

Value

Arguments

Author

References

Examples