sommer (version 1.6)

Technow_data: Genotypic and Phenotypic data from single cross hybrids (Technow et al. (2014))

Description

This dataset contains phenotpic data for 2 traits measured in 1254 single cross hybrids coming from the cross of Flint x Dent heterotic groups. In addition contains the genotipic data (35,478 markers) for each of the 123 Dent lines and 86 Flint lines. The purpose of this data is to demosntrate the prediction of unrealized crosses (9324 unrealized crosses, 1254 evaluated, total 10578 single crosses). We have added the additive relationship matrix (A) but can be easily obtained using the A.mat function on the marker data. Please if using this data for your own research cite Technow et al. (2014) publication (see References).

Usage

data("Technow_data")

format

The format is: chr "Technow_data"

source

This data was extracted from Technow et al. (2014).

References

If using this data for your own research please cite:

Technow et al. 2014. Genome properties and prospects of genomic predictions of hybrid performance in a Breeding program of maize. Genetics 197:1343-1355.

Examples

####=========================================####
#### For CRAN time limitations most lines in the 
#### examples are silenced with one '#' mark, 
#### remove them and run the examples
####=========================================####
data(Technow_data)

A.flint <- Technow_data$AF # Additive relationship matrix Flint
A.dent <- Technow_data$AD # Additive relationship matrix Dent
M.flint <- Technow_data$MF # Marker matrix Flint
M.dent <- Technow_data$MD # Marker matrix Dent

pheno <- Technow_data$pheno # phenotypes for 1254 single cross hybrids
pheno$hy <- paste(pheno$dent, pheno$flint, sep=":");head(pheno);dim(pheno) 

####=========================================####
#### CREATE A DATA FRAME WITH ALL POSSIBLE HYBRIDS
####=========================================####
#DD <- kronecker(A.dent,A.flint,make.dimnames=TRUE)

#hybs <- data.frame(sca=rownames(DD),yield=NA,matter=NA,gcad=NA, gcaf=NA)
#hybs$yield[match(pheno$hy, hybs$sca)] <- pheno$GY
#hybs$matter[match(pheno$hy, hybs$sca)] <- pheno$GM
#hybs$gcad <- as.factor(gsub(":.*","",hybs$sca))
#hybs$gcaf <- as.factor(gsub(".*:","",hybs$sca))
#head(hybs)
#dim(hybs)

####=========================================####
#### CREATE INCIDENCE MATRICES
####=========================================####
#Z1 <- model.matrix(~gcad-1, data=hybs); dim(Z1)
#Z2 <- model.matrix(~gcaf-1, data=hybs); dim(Z2)
#Z3 <- model.matrix(~sca-1, data=hybs); dim(Z3) #only full model
####=========================================####
#### SORT INCIDENCE MATRICES ACCORDING TO RELATIONSHIP MATRICES, REAL ORDERS
####=========================================####
#real1 <- match(  colnames(A.dent), gsub("gcad","",colnames(Z1)))
#real2 <- match(  colnames(A.flint), gsub("gcaf","",colnames(Z2)))
#real3 <- match(  colnames(DD), gsub("sca","",colnames(Z3)))
#Z1 <- Z1[,real1]
#Z2 <- Z2[,real2]
#Z3 <- Z3[,real3]
####=========================================####
#### RUN THE PREDICTION MODEL
####=========================================####
#ETA2 <- list(GCA1=list(Z=Z1, K=A.dent), GCA2=list(Z=Z2, K=A.flint)) 
#anss2 <- mmer(y=hybs$yield, Z=ETA2, method="AI") 
#summary(anss2)
#### try EM algorithm as well if only 2 variance components
#### you can try adding SCA effects by adding SCA=list(Z=Z3, K=DD)
#### to your Z parameter


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####=========================================####
####=========================================####
#### EXAMPLE 2
#### GWAS in single cross hybrids
####=========================================####
####=========================================####
data(Technow_data)

####=========================================####
#### RUN THE PREDICTION MODEL
####=========================================####
A.flint <- Technow_data$AF # Additive relationship matrix Flint
A.dent <- Technow_data$AD # Additive relationship matrix Dent

pheno <- Technow_data$pheno # phenotypes for 1254 single cross hybrids
pheno$hy <- paste(pheno$dent, pheno$flint, sep=":");head(pheno);dim(pheno) 

#DD <- kronecker(A.dent,A.flint,make.dimnames=TRUE)
#hybs <- data.frame(sca=rownames(DD),yield=NA,matter=NA,gcad=NA, gcaf=NA)
#hybs$yield[match(pheno$hy, hybs$sca)] <- pheno$GY
#hybs$matter[match(pheno$hy, hybs$sca)] <- pheno$GM
#hybs$gcad <- as.factor(gsub(":.*","",hybs$sca))
#hybs$gcaf <- as.factor(gsub(".*:","",hybs$sca))
#head(hybs)
####=========================================####
#### Get BLUPs for parental lines
####=========================================####
#mix3 <- mmer2(matter~1, random=~gcad + gcaf, method="EM", data=hybs)
#matterBLUP <- rbind(mix3$u.hat$gcad, mix3$u.hat$gcaf) 
####=========================================####
#### RUN GWAS combining markers
####=========================================####
#M.dent <- Technow_data$MD # Marker matrix Dent
#M.flint <- Technow_data$MF # Marker matrix Flint
#Mall <- rbind(M.dent,M.flint)

#AA <- A.mat(Mall) # Additive relationship matrix
#ZZ <- diag(dim(AA)[1]) # incidence matrix
#ETA <- list(sc=list(Z=ZZ, K=AA)) # Random component 
#mix4 <- mmer(y=matterBLUP, Z=ETA, W=Mall, method = "AI") # mixed model
#summary(mix4)