systemPipeR (version 1.6.2)

preprocessReads: Run custom read preprocessing functions

Description

Applies custom read preprocessing functions to single-end or paired-end FASTQ files. The function uses the FastqStreamer function from the ShortRead package to stream through large files in a memory-efficient manner.

Usage

preprocessReads(args, Fct, batchsize = 1e+05, overwrite = TRUE, ...)

Arguments

args

Object of class SYSargs

Fct

character string of custom read preprocessing function call where both the input and output needs to be an object of class ShortReadQ. The name of the input ShortReadQ object needs to be fq.

batchsize

Number of reads to process in each iteration by the internally used FastqStreamer function.

overwrite

If TRUE existing file will be overwritten.

...

To pass on additional arguments to the internally used writeFastq function.

Value

• Writes to files in FASTQ format. Their names are specified by outpaths(args).

See Also

FastqStreamer

Examples

## Preprocessing of single-end reads
param <- system.file("extdata", "trim.param", package="systemPipeR")
targets <- system.file("extdata", "targets.txt", package="systemPipeR")
args <- systemArgs(sysma=param, mytargets=targets)
preprocessReads(args=args, Fct="trimLRPatterns(Rpattern='GCCCGGGTAA', subject=fq)", batchsize=100000, overwrite=TRUE, compress=TRUE)

## Preprocessing of paired-end reads
param <- system.file("extdata", "trimPE.param", package="systemPipeR")
targets <- system.file("extdata", "targetsPE.txt", package="systemPipeR")
args <- systemArgs(sysma=param, mytargets=targets)
preprocessReads(args=args, Fct="trimLRPatterns(Rpattern='GCCCGGGTAA', subject=fq)", batchsize=100000, overwrite=TRUE, compress=TRUE)