twilight
objects. Produces one of five possible plots."plot"(x, which = NULL, grayscale = FALSE, legend = TRUE, ...)
which="scores"
plots the expected versus the observed test statistics and draws confidence lines calculated from permutations. This plot is similar to plots in Tusher et al. (2001).Option which="qvalues"
plots $q$-values versus the number of rejected hypotheses.
Option which="fdr"
plots $p$-values versus 1 - local false discovery rate, that is the conditional probability of being significant given the corresponding $p$-value, plus bootstrap estimates if computed. Bottom ticks are 1%-quantiles of $p$-values.
Option which="volcano"
results in the volcano plot, that is observed score versus 1 - local false discovery rate. Bottom ticks are 1%-quantiles of scores.
Option which="effectsize"
plots the observed fold change equivalent score distribution overlaid by the estimated effect size distribution, that is distribution of scores under the alternative. This plot is only available if function twilight.pval
was applied with method="fc"
as fold change equivalent scores are computed from log ratios.
Option which="table"
tabulates histogram "effectsize"
.
Scheid S and Spang R (2005): twilight; a Bioconductor package for estimating the local false discovery rate, Bioinformatics 21(12), 2921--2922.
Scheid S and Spang R (2006): Permutation filtering: A novel concept for significance analysis of large-scale genomic data, in: Apostolico A, Guerra C, Istrail S, Pevzner P, and Waterman M (Eds.): Research in Computational Molecular Biology: 10th Annual International Conference, Proceedings of RECOMB 2006, Venice, Italy, April 2-5, 2006. Lecture Notes in Computer Science vol. 3909, Springer, Heidelberg, pp. 338-347.
Tusher VG, Tibshirani R and Chu G (2001): Significance analysis of mircroarrays applied to the ionizing response, PNAS 98(9), 5116--5121.
twilight.pval
, twilight
### contains a twilight object created by function twilight
data(exfdr)
plot(exfdr)
Run the code above in your browser using DataCamp Workspace