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photobiology

Package ‘photobiology’ defines a system of classes for storing spectral data and accompanying metadata. For each of these classes specialised summary methods, maths operators and functions are provided. In addition, classes for storing collections of objects of the classes for individual spectra are defined as well as ‘apply’ functions. Extraction and replacement operators are implemented.

Functions for calculation of the position of the sun, times of sunrise and sunset, day length and night length are also available. Starting from ‘photobiology’ 0.12.0 they are imported from package ‘SunCalcMeeus’, which is a spin-off of ’photobiology. If you only use these functions, you can attach or load ‘SunCalcMeeus’ instead of ‘photobiology’, otherwise, the only visible change is that the on-line help is at a separate site.

Functions for the estimation of evapotranspiration and the energy balance of vegetation have been migrated to package ‘photobiologyPlants’ as they are related to vegetation. To use them with this version of package ‘photobiology’, please, add library(photobiologyPlants) to your code.

The package supports storage and manipulation of data for radiation quantities and for optical properties of objects and solutes, as well as response and action spectra for photobiological, photochemical and photoelectrical responses.

This package is the core of a suite of R packages for photobiological calculations described at the r4photobiology web site and in the vignette The R for Photobiology Suite.

Examples

The first example shows you how to estimate solar irradiance in W/m2 under a filter. We use a measured solar spectrum and a measured filter transmission spectrum.

library(photobiology)
e_irrad(sun.spct * yellow_gel.spct)
#> E_Total 
#> 146.506 
#> attr(,"time.unit")
#> [1] "second"
#> attr(,"radiation.unit")
#> [1] "total energy irradiance"

The second example shows some simple astronomical calculations for the sun.

geocode <- data.frame(lon = 0, lat = 55)
date <- lubridate::now(tzone = "UTC")
sunrise_time(date, tz = "UTC", geocode = geocode)
#> [1] "2025-09-22 05:46:15 UTC"
day_length(date, tz = "UTC", geocode = geocode)
#> [1] 12.21339

Installation

Installation of the most recent stable version from CRAN:

install.packages("photobiology")

Installation of the current unstable version from R-Universe CRAN-like repository:

install.packages('photobiology', 
                 repos = c('https://aphalo.r-universe.dev', 
                           'https://cloud.r-project.org'))

The two approaches above, automatically install dependencies.

Installation of the current unstable version from GitHub:

# install.packages("devtools")
devtools::install_github("aphalo/photobiology")

Installation from GitHub sources does not automatically install dependencies. Once package ‘photobiology’ is installed, installation of the remaining or missing packages in the suite from CRAN (or by adding the repository information as above, also from R-Universe):

intalled_pkgs <- installed.packages()[ , 1]
missing_pkgs <- setdiff(photobiology::r4p_pkgs, intalled_pkgs)
if (length(missing_pkgs) > 0) {
 install.packages(missing_pkgs)
}

Documentation

HTML documentation is available at (https://docs.r4photobiology.info/photobiology/), including two User Guides.

News on updates to the different packages of the ‘r4photobiology’ suite are regularly posted at (https://www.r4photobiology.info/).

Two articles introduce the basic ideas behind the design of the suite and describe its use: Aphalo P. J. (2015) (https://doi.org/10.19232/uv4pb.2015.1.14) and Aphalo P. J. (2016) (https://doi.org/10.19232/uv4pb.2016.1.15).

A book is under preparation, and the draft is currently available at (https://leanpub.com/r4photobiology/).

A handbook written before the suite was developed contains useful information on the quantification and manipulation of ultraviolet and visible radiation: Aphalo, P. J., Albert, A., Björn, L. O., McLeod, A. R., Robson, T. M., & Rosenqvist, E. (Eds.) (2012) Beyond the Visible: A handbook of best practice in plant UV photobiology (1st ed., p. xxx + 174). Helsinki: University of Helsinki, Department of Biosciences, Division of Plant Biology. ISBN 978-952-10-8363-1 (PDF), 978-952-10-8362-4 (paperback). PDF file available from (https://hdl.handle.net/10138/37558).

Contributing

Pull requests, bug reports, and feature requests are welcome at (https://github.com/aphalo/photobiology).

Citation

If you use this package to produce scientific or commercial publications, please cite according to:

citation("photobiology")
#> To cite package ‘photobiology’ in publications use:
#> 
#>   Aphalo, Pedro J. (2015) The r4photobiology suite. UV4Plants Bulletin,
#>   2015:1, 21-29. DOI:10.19232/uv4pb.2015.1.14
#> 
#> A BibTeX entry for LaTeX users is
#> 
#>   @Article{,
#>     author = {Pedro J. Aphalo},
#>     title = {The r4photobiology suite},
#>     journal = {UV4Plants Bulletin},
#>     volume = {2015},
#>     number = {1},
#>     pages = {21-29},
#>     year = {2015},
#>     doi = {10.19232/uv4pb.2015.1.14},
#>   }

License

© 2012-2025 Pedro J. Aphalo (pedro.aphalo@helsinki.fi). Released under the GPL, version 2 or greater. This software carries no warranty of any kind.

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Install

install.packages('photobiology')

Monthly Downloads

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Version

0.14.0

License

GPL (>= 2)

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Maintainer

Pedro Aphalo

Last Published

September 26th, 2025

Functions in photobiology (0.14.0)

FEL_spectrum

Incandescent "FEL" lamp emission spectrum
A2T

Convert absorbance into transmittance
D2.UV653

Data for typical calibration lamps
D2_spectrum

Calculate deuterium lamp output spectrum from fitted constants
Afr2T

Convert transmittance into absorptance.
MathFun

Miscellaneous Mathematical Functions
A.illuminant.spct

CIE A illuminant data
D50.illuminant.spct

CIE D50 illuminant data
D65.illuminant.spct

CIE D65 illuminant data
Ler_leaf.spct

Green Arabidopsis leaf reflectance and transmittance.
Rfr_from_n

Reflectance at a planar boundary
Rfr_normdiff

reflectance:reflectance normalised difference
Rfr_ratio

reflectance:reflectance ratio
Rfr_fraction

reflectance:reflectance fraction
Tfr_ratio

transmittance:transmittance ratio
Trig

Trigonometric Functions
Tfr_fraction

transmittance:transmittance fraction
Tfr_normdiff

transmittance:transmittance normalised difference
T2A

Convert transmittance into absorbance.
T2Afr

Convert transmittance into absorptance.
any2T

Convert filter quantities.
add_attr2tb

Copy attributes from members of a generic_mspct
absorbance

Absorbance
absorptance

Absorptance
adaptive_smoothing

Custom smoothing
as.calibration_spct

Coerce to a spectrum
as.calibration_mspct

Coerce to a collection-of-spectra
absorptance_spct

Calculate absorptance from spectral absorptance.
absorbance_spct

Calculate absorbance from spectral absorbance.
as.cps_mspct

Coerce to a collection-of-spectra
as.filter_mspct

Coerce to a collection-of-spectra
as.cps_spct

Coerce to a spectrum
as.chroma_spct

Coerce to a spectrum
as.object_spct

Coerce to a spectrum
as.filter_spct

Coerce or convert into a filter spectrum
as.object_mspct

Coerce to a collection-of-spectra
as.generic_spct

Coerce to a spectrum
as.matrix-mspct

Coerce a collection of spectra into a matrix
as.generic_mspct

Coerce to a collection-of-spectra
as.source_spct

Coerce to a spectrum
as.source_mspct

Coerce to a collection-of-spectra
as.raw_spct

Coerce to a spectrum
as.raw_mspct

Coerce to a collection-of-spectra
as_quantum_mol

Convert spectral energy irradiance into spectral photon irradiance
auto_hinges

Guess whether insertion of hinges is needed or not
as.response_mspct

Coerce to a collection-of-spectra
as.response_spct

Coerce to a spectrum
calc_source_output

Scaled and/or interpolated light-source spectral output
calc_multipliers

Spectral weights
checkSpctVersion

Check that the "spct.version" attribute is set
as_quantum

Convert spectral energy irradiance into spectral photon irradiance
as.solute_mspct

Coerce to a collection-of-spectra
as.chroma_mspct

Coerce to a collection-of-spectra
checkTimeUnit

Check the "time.unit" attribute of an existing source_spct object
as_energy

Convert spectral photon irradiance into spectral energy irradiance
check_wl_stepsize

Check consistency of wavelength step size
c

Combine collections of spectra
as.solute_spct

Coerce to a solute spectrum
black_body.spct

Theoretical optical bodies
average_spct

Average spectral data.
beesxyzCMF.spct

Honeybee xyz chromaticity colour matching function data
ciev10.spct

Linear energy CIE 2008 luminous efficiency function 10 deg data
ciexyzCMF10.spct

Linear energy CIE xyz colour matching function (CMF) 10 deg data
check_w.length

Sanity check of wavelengths (internal function).
check_spectrum

Sanity check a spectrum
check_and_rename_vars

check and rename variables
ciexyzCC2.spct

CIE xyz chromaticity coordinates 2 deg data
ciev2.spct

Linear energy CIE 2008 luminous efficiency function 2 deg data
as.reflector_spct

Coerce to a spectrum
clean

Clean (=replace) off-range values in a spectrum
as.reflector_mspct

Coerce to a collection-of-spectra
check_spct

Check validity of spectral objects
clear_photobio.cache

Clear the spectral weights cache
ccd.spct

Spectral response of a back-thinned CCD image sensor.
checkMspctVersion

Check that the "mspct.version" attribute is set
clean_spct

Clean a spectrum
ciexyzCC10.spct

CIE xyz chromaticity coordinates (CC) 10 deg data
clear.spct

Theoretical spectrum of clear and apaque materials
class_spct

Query which is the class of a spectrum
ciexyzCMF2.spct

Linear energy CIE xyz colour matching function (CMF) 2 deg data
convertThickness

Convert the "thickness" attribute of an existing filter_spct object.
color_of

Color of an object
compare_spct

Coarse-grained comparison of two spectra
cone_fundamentals10.spct

Ten-degree cone fundamentals
collect2mspct

Form a new collection
convertTfrType

Convert the "Tfr.type" attribute
clip_wl

Clip head and/or tail of a spectrum
copy_attributes

Copy attributes
convolve_each

Convolve function for collections of spectra
convertTimeUnit

Convert the "time.unit" attribute of an existing source_spct object
div-.generic_spct

Arithmetic Operators
div_spectra

Divide two spectra, even if the wavelengths values differ
diffraction_single_slit

Diffraction
drop_user_cols

Drop user columns
dim.generic_mspct

Dimensions of an Object
cps2irrad

Conversion from counts per second to physical quantities
defunct

Defunct functions and methods
e2q

Convert energy-based quantities into photon-based quantities.
despike

Remove spikes from spectrum
denormalize_spct

Remove a previously applied normalization
e_response

Energy-based photo-response
enable_check_spct

Enable or disable checks
e_fraction

Energy:energy fraction
e_fluence

Energy fluence
energy_as_default

Set spectral-data options
energy_irradiance

Calculate (energy) irradiance from spectral irradiance
energy_ratio

Energy:energy ratio
eq_ratio

Energy:photon ratio
spread

Expanse
Extract

Extract or replace parts of a spectrum
e2qmol_multipliers

Calculate energy to quantum (mol) multipliers
e2quantum_multipliers

Calculate energy to quantum multipliers
Extract_mspct

Extract or replace members of a collection of spectra
f_dispatcher_spct

Math function dispatcher for spectra
fscale_spct

fscale a spectrum
e_ratio

Energy:energy ratio
e_irrad

Energy irradiance
fscale

Rescale a spectrum using a summary function
findMultipleWl

Find repeated w.length values
fshift

Shift the scale of a spectrum using a summary function
fshift_spct

fshift a spectrum
filter_cps.mspct

Counts per second from a measurement of a plastic film
getInstrDesc

Get the "instr.desc" attribute
getInstrSettings

Get the "instr.settings" attribute
getSoluteProperties

Get the "solute.properties" attribute
getScaled

Get the "scaled" attribute
getFilterProperties

Get the "filter.properties" attribute
generic_mspct

Collection-of-spectra constructor
getIdFactor

Get the "idfactor" attribute
getHowMeasured

Get the "how.measured" attribute
getWhereMeasured

Get the "where.measured" attribute
get_attributes

Get the metadata attributes
getSpctVersion

Get the "spct.version" attribute
getTimeUnit

Get the "time.unit" attribute of an existing source_spct object
find_wls

Find wavelength values in a spectrum
fit_peaks

Refine position and value of extremes by fitting
find_spikes

Find spikes
find_peaks

Find local maxima or global maximum (peaks)
green_leaf.spct

Green birch leaf reflectance.
get_peaks

Get peaks and valleys from a spectrum
getMspctVersion

Get the "mspct.version" attribute
insert_hinges

Insert wavelength values into spectral data.
getKType

Get the "K.type" attribute
integrate_xy

Gives irradiance from spectral irradiance.
getWhatMeasured

Get the "what.measured" attribute
getWhenMeasured

Get the "when.measured" attribute
integrate_spct

Integrate spectral data.
fluence

Fluence
is_absorbance_based

Query if a spectrum contains absorbance or transmittance data
is_effective

Is an R object "effective"
isValidInstrDesc

Check the "instr.desc" attribute
isValidInstrSettings

Check the "instr.settings" attribute
is_mole_based

Query if a spectrum contains mole or mass based data
interpolate_spct

Map a spectrum to new wavelength values.
interpolate_spectrum

Calculate spectral values at a different set of wavelengths
head_tail

Return the First and Last Parts of an Object
formatted_range

Compute range and format it
interpolate_wl

Map spectra to new wavelength values.
getMultipleWl

Get the "multiple.wl" attribute
getNormalized

Query the "normalized" and "normalization" attributes
illuminance

Irradiance
irradiance

Photon or energy irradiance from spectral energy or photon irradiance.
is.generic_spct

Query class of spectrum objects
is.generic_mspct

Query class of spectrum objects
is_normalized

Query whether a generic spectrum has been normalized.
is_tagged

Query if a spectrum is tagged
join_mspct

Join all spectra in a collection
insert_spct_hinges

Insert new wavelength values into a spectrum
is.summary_generic_spct

Query class of spectrum summary objects
make_var_labels

Column or variable labels
is.waveband

Query if it is a waveband
make_wl_consistent

Make wavelengths consistent
labels

Find labels from "waveband" object
log

Logarithms and Exponentials
msmsply

Multi-spct transform methods
mod-.generic_spct

Arithmetic Operators
merge_attributes

Merge and copy attributes
wl_midpoint

Midpoint
is.old_spct

Query if an object has old class names
wl_max

Wavelength maximum
is_photon_based

Query if a spectrum contains photon- or energy-based data.
is_scaled

Query whether a generic spectrum has been scaled
peaks

Peaks or local maxima
minus-.generic_spct

Arithmetic Operators
oper_spectra

Binary operation on two spectra, even if the wavelengths values differ
wl_min

Wavelength minimum
normalization

Normalization of an R object
normalize

Normalize spectral data
irrad

Irradiance
normalize_range_arg

Normalize a range argument into a true numeric range
normalized_diff_ind

Calculate a normalized difference.
merge2object_spct

Merge into object_spct
photon_ratio

Photo:photon ratio
photons_energy_ratio

Photon:energy ratio
mspct_classes

Names of multi-spectra classes
l_insert_hinges

Insert wavelength values into spectral data.
na.omit

Handle Missing Values in Objects
phenylalanine.spct

Molar spectral attenuation coefficient of phenylalanine
calendar_change

Solar astronomy using Meeus' algorithm
^.generic_spct

Arithmetic Operators
plus-.generic_spct

Arithmetic Operators
pull_sample

Random sample of spectra
photobiology-package

photobiology: Photobiological Calculations
q_fluence

Photon fluence
q2e

Convert photon-based quantities into energy-based quantities
print.summary_generic_spct

Print spectral summary
prod_spectra

Multiply two spectra, even if the wavelengths values differ
print.waveband

Print a "waveband" object
photon_irradiance

Photon irradiance
photodiode.spct

Spectral response of a GaAsP photodiode
wl_range

Wavelength range
rmDerivedSpct

Remove "generic_spct" and derived class attributes.
range_check_Tfr

Internal utility range-check functions
round

Rounding of Numbers
replace_bad_pixs

Replace bad pixels in a spectrum
reflectance_spct

Calculate reflectance from spectral reflectance
print.generic_spct

Print spectral objects
q_irrad

Photon irradiance
print.metadata

Print methods for metadata records
q_fraction

Photon:photon fraction
s_mean_se_band

Mean plus and minus standard error from collection of spectra
s_median

Median of a collection of spectra
rowwise

rowwise functions for collections of spectra
qe_ratio

Photon:energy ratio
response

Integrated response
rgb_spct

RGB color values
resp_spct

Calculate response from spectral response
r4p_pkgs

Packages in R for Photobiology suite
rbindspct

Row-bind spectra
s_mean

Mean from collection of spectra
reflectance

Reflectance
q_ratio

Photon:photon ratio
q_response

Photon-based photo-response
rmDerivedMspct

Remove "generic_mspct" and derived class attributes.
se.m

Standard error of the mean
se.p

Standard error of the mean
s_se

Standard Error of a collection of spectra
setNormalized

Set the "normalized" and "normalization" attributes
s_range

Range of a collection of spectra
s_sd

Standard Deviation of a collection of spectra
select_spct_attributes

Merge user supplied attribute names with default ones
setBSWFUsed

The "bswf.used" attribute
s_prod

Product from collection of spectra
s_var

Variance of a collection of spectra
s_quantile

Quantiles of a collection of spectra
se

Standard error of the mean
setIdFactor

Set the "idfactor" attribute
setHowMeasured

Set the "how.measured" attribute
s_e_irrad2rgb

Spectral irradiance to rgb color conversion
setFilterProperties

Set the "filter.properties" attribute
setGenericSpct

Convert an R object into a spectrum object.
setResponseType

Set the "response.type" attribute
s_mean_se

Mean and standard error from collection of spectra
setScaled

Set the "scaled" attribute
sinc

sinc
setRfrType

The "Rfr.type" attribute
setTimeUnit

Set the "time.unit" attribute of an existing source_spct object
s_sum

Sum from collection of spectra
setInstrDesc

Set the "instr.desc" attribute
setInstrSettings

Set the "instr.settings" attribute
slash-.generic_spct

Arithmetic Operators
shared_member_class

Classes common to all collection members.
sign

Sign
setTfrType

The "Tfr.type" attribute
setKType

Set the "K.type" attribute
setSoluteProperties

Set the "solute.properties" attribute
setMultipleWl

Set the "multiple.wl" attribute
setWhatMeasured

Set the "what.measured" attribute
spct_attr2tb

Copy attributes into a tibble
split2mspct

Convert a 'wide' or untidy data frame into a collection of spectra
setWhenMeasured

Set the "when.measured" attribute
spct_wide2long

Convert spectrum from wide to long form
spct_metadata

Access metadata
setWhereMeasured

Set the "where.measured" attribute
spct_classes

Function returning a vector containing the names of spectra classes.
spikes

Spikes
smooth_spct

Smooth a spectrum
source_spct

Spectral-object constructors
split_energy_irradiance

Energy irradiance for split spectrum regions
split_bands

List-of-wavebands constructor
wl_stepsize

Stepsize
subset2mspct

Convert 'long' or tidy spectral data into a collection of spectra
Subset

Subsetting spectra
subt_spectra

Subtract two spectra
split_photon_irradiance

Photon irradiance for split spectrum regions
subset_attributes

Subset the metadata attributes
summary_spct_classes

Function that returns a vector containing the names of spectral summary classes.
sum_spectra

Add two spectra
summary.generic_spct

Summary of one or more spectra
split_irradiance

Energy or photon irradiance for split spectrum regions
trimInstrSettings

Trim the "instr.settings" attribute
transmittance_spct

Calculate transmittance from spectral transmittance.
thin_wl

Thin the density of wavelength values
times-.generic_spct

Arithmetic Operators
transmittance

Transmittance
sun_daily.spct

Daily solar spectral irradiance (simulated)
sun.spct

Solar spectral irradiance (simulated)
trimInstrDesc

Trim the "instr.desc" attribute
two_reflectances

Compute two reflectances for ratio, fraction or normalised difference
trim_spct

Trim (or expand) head and/or tail of a spectrum
trim_tails

Trim (or expand) head and/or tail
two_sensors.mspct

Spectral response of two light sensors.
tag

Tag a spectrum
two_filters.spct

Transmittance spectrum of plastic films
sun_evening.spct

Time series of solar spectral irradiance (measured)
two_irrads

Compute two irrads for ratio, fraction or normalised difference
uncollect2spct

Extract all members from a collection
trim_wl

Trim head and/or tail of a spectrum
trim_waveband

Trim (or expand) head and/or tail
two_transmittances

Compute two transmittances for ratio, fraction or normalised difference
v_insert_hinges

Insert spectral data values at new wavelength values.
using_Tfr

Use photobiology options
upgrade_spectra

Upgrade one or more spectral objects
v_replace_hinges

Overwrite spectral data values at existing wavelength values.
w_length_range2rgb

Wavelength range to rgb color conversion
untag

Remove tags
verbose_as_default

Set error reporting options
valleys

Valleys or local minima
upgrade_spct

Upgrade one spectral object
w_length2rgb

Wavelength to rgb color conversion
wb2spct

Create spectrum from wavebands
white_led.source_spct

White led bulb spectrum
waveband

Waveband constructor method
wls_at_target

Find wavelengths values corresponding to a target spectral value
wl2wavenumber

Wavelength conversions
wb2rect_spct

Create tagged spectrum from wavebands
water.spct

Molar spectral attenuation coefficient of water
wb_trim_as_default

Set computation options
waveband_ratio

Photon or energy ratio
wb2tagged_spct

Create tagged spectrum from wavebands