seqinr (version 1.0-6)

oriloc: Prediction of origin and terminus of replication in bacteria

Description

This program finds the putative origin and terminus of replication in procaryotic genomes. The program discriminates between codon positions.

Usage

oriloc(seq.fasta = system.file("sequences/ct.fasta", package ="seqinr"),
 g2.coord = system.file("sequences/ct.coord", package = "seqinr"),
oldoriloc = FALSE, gbk = NULL, clean.tmp.files = TRUE, rot = 0)

Arguments

seq.fasta

the name of a file which contains the dna sequence of a bacterial chromosome in fasta format

g2.coord

the name of file which contains the output of glimmer2 program

oldoriloc

logical to be set at TRUE to reproduce the (deprecated) outputs of previous (publication date: 2000) version of the oriloc program

gbk

the URL of a file in GenBank format

clean.tmp.files

Logical, if TRUE temporary files are removed

rot

Integer, with zero default value, used to permute circurlarly the genome.

Value

• A data.frame with seven columns: g2num for the CDS number in the g2.coord file, start.kb for the start position of CDS expressed in Kb (this is the position of the first occurence of a nucleotide in a CDS regardless of its orientation), end.kb for the last position of a CDS, CDS.excess for the DNA walk for gene orientation (+1 for a CDS in the direct strand, -1 for a CDS in the reverse strand) cummulated over genes, skew for the cummulated composite skew in third codon positions, x for the cummulated T - A skew in third codon position, y for the cummulated C - G skew in third codon positions.

Details

The method builds on the fact that there are compositional asymmetries between the leading and the lagging strand for replication. The programs works only with third codon positions so as to increase the signal/noise ratio. To discriminate between codon positions, the program use as input either an annotated genbank file, either a fasta file and a glimmer2.0 output file.

References

More illustrated explanations to help understand oriloc outputs are available there: http://pbil.univ-lyon1.fr/software/Oriloc/howto.html. Examples of oriloc outputs on real sequence data are there: http://pbil.univ-lyon1.fr/software/Oriloc/index.html. The original paper for oriloc: Frank, A.C., Lobry, J.R. (2000) Oriloc: prediction of replication boundaries in unannotated bacterial chromosomes. Bioinformatics, 16:566-567. http://bioinformatics.oupjournals.org/cgi/reprint/16/6/560 A simple informal introduction to DNA-walks: Lobry, J.R. (1999) Genomic landscapes. Microbiology Today, 26:164-165. http://www.socgenmicrobiol.org.uk/QUA/049906.pdf An early and somewhat historical application of DNA-walks: Lobry, J.R. (1996) A simple vectorial representation of DNA sequences for the detection of replication origins in bacteria. Biochimie, 78:323-326. Glimmer, a very efficient open source software for the prediction of CDS from scratch in prokaryotic genome, is decribed at http://www.cbcb.umd.edu/software/glimmer/. citation("seqinr")

Examples

# A little bit too long for routine checks:
library(ade4)
out <- oriloc()
plot(out$st, out$sk, type = "l", xlab = "Map position in Kb",
    ylab = "Cumulated composite skew", 
    main = expression(italic(Chlamydia~~trachomatis)~~complete~~genome))