RJaCGH (version 1.1.1)

pMCR: Probabilistic Minimal Common Regions for copy number alteration.

Description

This method compute regions of gain/lost copy number with a joint probability of alteration greater than a given threshold.

Usage

pMCR(obj, p, alteration = "Gain", array.weights = NULL)
## S3 method for class 'RJaCGH':
pMCR(obj, p, alteration = "Gain", array.weights = NULL)
## S3 method for class 'RJaCGH.Chrom':
pMCR(obj, p, alteration = "Gain", array.weights = NULL)
## S3 method for class 'RJaCGH.genome':
pMCR(obj, p, alteration = "Gain", array.weights
= NULL)
## S3 method for class 'RJaCGH.array':
pMCR(obj, p, alteration = "Gain", array.weights = NULL)

Arguments

obj

An object of class 'RJaCGH', 'RJaCGH.Chrom', 'RJaCGH.genome' or 'RJaCGH.array'.

p

Threshold for the minimum joint probability of alteration of the region.

alteration

Either 'Gain' or 'Lost'

array.weights

When 'obj' contains several arrays, the user can give a weight to each of them according to their reliability or precision.

Value

• An object of class pMCR.RJaCGH, pMCR.RJaCGH.Chrom or RJaCGH.genome, as corresponding. They are lists with a sublist for every region encountered and elements:
• start
  Start position of the region.
• indexStart
  index position of the start of the region.
• indexEnd
  index position of the end of the region.
• end
  End position of the region.
• genes
  Number of genes in the region.
• prob
  Joint probability of gain/loss of the region.
• If there are chromosome information (that is, the object inputed is of class RJaCGH.Chrom, RJaCGH.genome or RJaCGH.array with each array of any of these classes), then those information will be enclosed in a list for each chromosome.

Details

RJaCGH can compute minimal common regions taking into account the probability of every gene to have an altered copy number. The result is a set of genes whose joint probability (not the product of their marginal probabilities, as returned by 'states' or 'model.averaging' is at least as 'p' or greater.

Please note that if the method returns several sets or regions, the probability of alteration of all of them doesn't have to be over the probability threshold; in other words 'p' is computed for every region, not for all the sequence of regions.

References

Oscar M. Rueda and Ramon Diaz Uriarte. A flexible, accurate and extensible statistical method for detecting genomic copy-number changes. http://biostats.bepress.com/cobra/ps/art9/ {http://biostats.bepress.com/cobra/ps/art9/}.

See Also

RJaCGH, states, model.averaging, print.pMCR.RJaCGH

Examples

## MCR for a single array:
y <- c(rnorm(100, 0, 1), rnorm(10, -3, 1), rnorm(20, 3, 1),
       rnorm(100,0, 1)) 
Pos <- sample(x=1:500, size=230, replace=TRUE)
Pos <- cumsum(Pos)
Chrom <- rep(1:23, rep(10, 23))

jp <- list(sigma.tau.mu=rep(0.05, 4), sigma.tau.sigma.2=rep(0.03, 4),
           sigma.tau.beta=rep(0.07, 4), tau.split.mu=0.1, tau.split.beta=0.1)

fit.genome <- RJaCGH(y=y, Pos=Pos, Chrom=Chrom, model="genome",
burnin=1000, TOT=1000, jump.parameters=jp, k.max = 4)
pMCR(fit.genome, p=0.8, alteration="Gain")
pMCR(fit.genome, p=0.8, alteration="Loss")

##MCR for two arrays:
z <- c(rnorm(110, 0, 1), rnorm(20, 3, 1),
       rnorm(100,0, 1)) 
fit.array.genome <- RJaCGH(y=cbind(y,z), Pos=Pos, Chrom=Chrom, model="genome",
burnin=1000, TOT=1000, jump.parameters=jp, k.max = 4)
pMCR(fit.array.genome, p=0.4, alteration="Gain")
pMCR(fit.array.genome, p=0.4, alteration="Loss")