filter_debris_flow

Detect events in the lower left corner of FSC-A/SSC-A plots.
This function aims for high specificity, but not high sensitivity: for
Cleanet's purposes, it suffices to deplete debris, even if not all of
it is eliminated.

Automated method for doublet detection in flow or mass cytometry data, based on simulating doublets and finding events whose protein expression patterns are similar to the simulated doublets.

Matei Ionita

Cleanet

Automated Doublet Detection and Classification for Cytometry
Data

filter_debris_flow function

<dl><dt>df</dt>
<dd>A data frame containing scattering channels and
protein expression data.</dd>
<dt>sum_max</dt>
<dd>Numeric; events whose sum of FSC-A and SSC-A is smaller than
this value are flagged.</dd>
<dt>cols</dt>
<dd>Names of columns to use. This function is intended for use
with the area channel of forward and side scatter.</dd></dl>

Arguments

Flag debris in flow cytometry data. — filter_debris_flow

<dl>

<dt>df</dt>
<dd>A data frame containing scattering channels and
protein expression data.</dd>


<dt>sum_max</dt>
<dd>Numeric; events whose sum of FSC-A and SSC-A is smaller than
this value are flagged.</dd>


<dt>cols</dt>
<dd>Names of columns to use. This function is intended for use
with the area channel of forward and side scatter.</dd>

</dl>

filter_debris_flow: Flag debris in flow cytometry data.

Description

Usage

Value

Arguments