EasyqpcR-package

EasyqpcR


This package contains functions to analyse real-time quantitative PCR data at
 IRTOMIT-INSERM U1082. The algorithm used is the one published by Hellemans et
 al. (2007). It permits the calculation of the normalization and calibration
 factors, the relative quantities, the normalized relative quantities which can
 then be scaled to your control group.


qPCR analysis

This package is based on the qBase algorithms published by
Hellemans et al. in 2007. The EasyqpcR package allows you to import easily qPCR
data files as described in the vignette. Thereafter, you can calculate
amplification efficiencies, relative quantities and their standard errors,
normalization factors based on the best reference genes choosen (using the
SLqPCR package), and then the normalized relative quantities, the NRQs scaled
to your control and their standard errors. This package has been created for
low-throughput qPCR data analysis.

Le Pape Sylvain

EasyqpcR for low-throughput real-time quantitative PCR data
analysis

EasyqpcR-package function

This package contains functions to analyse real-time quantitative PCR data at
 IRTOMIT-INSERM U1082. The algorithm used is the one published by Hellemans et
 al. (2007). It permits the calculation of the normalization and calibration
 factors, the relative quantities, the normalized relative quantities which can
 then be scaled to your control group.

EasyqpcR-package: Functions to analyse real-time quantitative PCR data at IRTOMIT-INSERM U1082

Description

Arguments

Details

References