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EasyqpcR (version 1.14.0)

EasyqpcR for low-throughput real-time quantitative PCR data analysis

Description

This package is based on the qBase algorithms published by Hellemans et al. in 2007. The EasyqpcR package allows you to import easily qPCR data files as described in the vignette. Thereafter, you can calculate amplification efficiencies, relative quantities and their standard errors, normalization factors based on the best reference genes choosen (using the SLqPCR package), and then the normalized relative quantities, the NRQs scaled to your control and their standard errors. This package has been created for low-throughput qPCR data analysis.

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Version

Version

1.14.0

License

GPL (>=2)

Maintainer

Le Pape Sylvain

Last Published

February 15th, 2017

Functions in EasyqpcR (1.14.0)

slope

Function to calculate the amplification efficiency
nrmData

Determination of the NF, RQ, NRQ, NRQ scaled to control and their SE and SD.
badCt

Evaluation of the qPCR technical replicates
EasyqpcR-package

Functions to analyse real-time quantitative PCR data at IRTOMIT-INSERM U1082
qPCR_run3

Raw data from the third qPCR run.
totData

Aggregation of qPCR biological replicates and data transformation
calData

Calculation of calibration factors
Gene_maximisation

A data frame containing all the data from four different qPCR runs.
qPCR_run1

Raw data from the first qPCR run.
qPCR_run2

Raw data from the second qPCR run.
Gene_maximisation_cor

A data frame containing all the data from four different qPCR runs.
Efficiency_calculation

Raw data for primer amplification efficiency calculation.