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GUIDEseq (version 1.2.1)

GUIDE-seq analysis pipeline

Description

The package implements GUIDE-seq analysis workflow including functions for obtaining unique insertion sites (proxy of cleavage sites), estimating the locations of the insertion sites, aka, peaks, merging estimated insertion sites from plus and minus strand, and performing off target search of the extended regions around insertion sites.

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Version

Version

1.2.1

License

GPL (>= 2)

Maintainer

Lihua Julie Zhu

Last Published

February 15th, 2017

Functions in GUIDEseq (1.2.1)

offTargetAnalysisOfPeakRegions

Offtarget Analysis of GUIDE-seq peaks
GUIDEseqAnalysis

Analysis pipeline for GUIDE-seq dataset
getPeaks

Obtain peaks from GUIDE-seq
GUIDEseq-package

Analysis of GUIDE-seq
uniqueCleavageEvents

example unique cleavage sites
getUniqueCleavageEvents

Using UMI sequence to obtain the starting sequence library
mergePlusMinusPeaks

Merge peaks from plus strand and minus strand
peaks.gr

example cleavage sites
combineOfftargets

Combine Offtargets