MBmca (version 0.0.3-2)

DMP: Bimodal melting curve experiment on the surface of microbeads.

Description

A melting curve experiment with six microbead populations and short oligonucleotide probes (direct hybridization). Detection probes for human VIM (vimentin), MLC-2v (myosin regulatory light chain 2, ventricular/cardiac muscle isoform), SERCA2 (Atp2a2 - ATPase, Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform), HRPT1 (hyperparathyroidism 1) and the artificial sequences Poly(dA)20 (20 nt of dA) and aCS (artificial Control Sequence).

Usage

data(DMP)

source

Data were measured with the VideoScan platform:

A Highly Versatile Microscope Imaging Technology Platform for the Multiplex Real-Time Detection of Biomolecules and Autoimmune Antibodies. S. Roediger, P. Schierack, A. Boehm, J. Nitschke, I. Berger, U. Froemmel, C. Schmidt, M. Ruhland, I. Schimke, D. Roggenbuck, W. Lehmann and C. Schroeder. Advances in Biochemical Bioengineering/Biotechnology. 133:35--74, 2013. http://www.ncbi.nlm.nih.gov/pubmed/22437246

Surface Melting Curve Analysis with R. S. Roediger, A. Boehm and I. Schimke. The R Journal. 5(2):37--52, 2013. http://journal.r-project.org/

See Also

MFIerror, mcaSmoother, diffQ, diffQ, MultiMelt, DualHyb

Examples

data(DMP)