MBmca (version 0.0.3-2)

MultiMelt: Surface melting curve data from direct hybridization experiment of short oligonucleotides.

Description

A melting curve experiment with twelve microbead populations and the short oligonucleotide capture probe and detection probe for human HRPT1 (hyperparathyroidism 1) and MLC-2v (myosin regulatory light chain 2, ventricular/cardiac muscle isoform).

Usage

data(MultiMelt)

source

Data were measured with the VideoScan platform:

A Highly Versatile Microscope Imaging Technology Platform for the Multiplex Real-Time Detection of Biomolecules and Autoimmune Antibodies. S. Roediger, P. Schierack, A. Boehm, J. Nitschke, I. Berger, U. Froemmel, C. Schmidt, M. Ruhland, I. Schimke, D. Roggenbuck, W. Lehmann and C. Schroeder. Advances in Biochemical Bioengineering/Biotechnology. 133:35--74, 2013. http://www.ncbi.nlm.nih.gov/pubmed/22437246

Surface Melting Curve Analysis with R. S. Roediger, A. Boehm and I. Schimke. The R Journal. 5(2):37--52, 2013, 2013. http://journal.r-project.org/

Details

The melting curve was conducted with short oligonucleotide probes on the surface of microbeads using the VideoScan platform according to Roediger et al. (2012). The dyes and quencher used were Atto 647N and BHQ2.

See Also

MFIerror, mcaSmoother, diffQ, diffQ2, DMP, DualHyb

Examples

data(MultiMelt)