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The MBmca package provides data sets and lightweight utilities for nucleic acid melting curve analysis and presentation on microbead surfaces.

Installation

MBmca is available on CRAN, so installation is as simple as:

install.packages("MBmca")

You can install the latest development version of the code using the devtools R package.

# Install devtools, if you haven't already.
install.packages("devtools")

library(devtools)
install_github("MBmca", "michbur")

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Version

Install

install.packages('MBmca')

Monthly Downloads

275

Version

0.0.3-5

License

GPL (>= 2)

Issues

0

Pull Requests

0

Stars

4

Forks

0

Maintainer

Stefan Roediger

Last Published

January 13th, 2015

Functions in MBmca (0.0.3-5)

DMP

Bimodal melting curve experiment on the surface of microbeads.
MBmca-package

Microbead Surface Nucleic Acid Melting Curve Analysis
MultiMelt

Surface melting curve data from direct hybridization experiment of short oligonucleotides.
diffQ

Calculation of the melting temperature (Tm) from the first derivative
MFIerror

Multiple comparison of the temperature dependent variance of the refMFI
mcaPeaks

Function to estimate the approximate local minima and maxima of melting curve data.
diffQ2

Calculation of the melting temperatures (Tm, Tm1D2 and Tm2D2) from the first and the second derivative
DualHyb

Surface melting curve data from direct hybridization experiment of short oligonucleotide probes with bimodal melting curve pattern.
mcaSmoother

Function to pre-process melting curve data.