NanoStringNorm (version 1.0.0)

predict.concentration: predict.concentration

Description

A function to predict the concentration of endogenous genes from their counts. The model is based on the relationship between the positive control counts and their known concentration.

Usage

predict.concentration(x, anno, log, verbose = TRUE);

Arguments

x

A NanoString RCC worksheet. If anno is not specified then the first three columns must be c("Code.Class","Name","Accession"). The remaining columns are samples and rows are genes.

anno

An alternative and flexible annotation dataset. The columns c("Code.Class","Name","Accession") do not need to be included in "x" if anno is specified.

log

Has the data been logged (log2). Raw NanoString data is not in log space. This will only effect the calculation of Fold-change.

verbose

Output run-time status messages

Value

• A matrix including t-test p-values and Fold-Changes for all genes and traits.