If type is "Bscore" (default) a Bscore-normalization is performed. If type is "spatial", a locfit regression is estimated that accounts for spatial effects.
maxit
Maximum number of iterations for locfit.
verbose
Either 0 (default, no output), 1 (minimum output), or 2 (outout).
Value
An object of class RNAinteract. The returned object contains the normalization information.
Details
The Bscore normalization estimates row and column effects for each plate. It returns the residuals to the sum of row and column effects. The spatial normalization estimates a non-linear 2D regression for each plate and returns the residuals.