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Ringo (version 1.36.0)
nimblegenScale: Function to compute scaled log-ratios
Description
This function compute the scaled log-ratios from raw probe intensities, as done by Nimblegen for scaling of ChIP-chip data.
Usage
nimblegenScale(myRG, ...)
Arguments
myRG
Object of class
RGList
...
further arguments passed on to
tukey.biweight
Value
Return an
MAList
, with the M slot of the list holding the scaled log ratios.
Details
Nimblegen provides scaled log-ratios as normalized values for the probes. log.ratio = log2(R)-log2(G) scaled.ratio = log.ratio - tukey.biweight(log.ratio)
References
for more details on the Tukey biweight estimator: Statistical Algorithms Description Document, 2002, Affymetrix.