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Ringo (version 1.36.0)

R Investigation of ChIP-chip Oligoarrays

Description

The package Ringo facilitates the primary analysis of ChIP-chip data. The main functionalities of the package are data read-in, quality assessment, data visualisation and identification of genomic regions showing enrichment in ChIP-chip. The package has functions to deal with two-color oligonucleotide microarrays from NimbleGen used in ChIP-chip projects, but also contains more general functions for ChIP-chip data analysis, given that the data is supplied as RGList (raw) or ExpressionSet (pre- processed). The package employs functions from various other packages of the Bioconductor project and provides additional ChIP-chip-specific and NimbleGen-specific functionalities.

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Version

Version

1.36.0

License

Artistic-2.0

Maintainer

J Toedling

Last Published

February 15th, 2017

Functions in Ringo (1.36.0)

image.RGList

Function to visualize spatial distribution of raw intensities
cherByThreshold

Function to identify chers based on thresholds
exportCherList

Function to export cherList into a file
regionOverlap

Function to compute overlap of genomic regions
chipAlongChrom

Visualize ChIP intensities along the chromosome
features2Probes

Function for mapping genomic features to probes
plot.autocor.result

Plots auto-correlation of probe intensities
readNimblegen

Function to read in Nimblegen Intensity Text Files
sigGOTable

Obtain significant GO terms for a list of genes
relateChers

Relate found Chers to genomic features
sliding.quantile

Compute quantile of scores in a sliding window
asExprSet

converts a Ringo MAList into an ExpressionSet
probeAnno-class

Class "probeAnno"
upperBoundNull

function to estimate upper limit of null distribution
twoGaussiansNull

Estimate a threshold from Gaussian mixture distribution
computeRunningMedians

Function to compute running medians on a tiling expression set
chipAlongChrom1

Visualize ChIP intensities along the chromosome
qop-class

Class "qop" Quantiles Over Positions
ftr2xys

Convert a NimbleScan ftr-file into a xys-file
computeSlidingT

Function to compute sliding T statistics on a tiling expression set
getFeats

Utility function to extract all features from a cherList
newCher

Create a list object of class cher
plot.cher

Plot identified Chers
corPlot

Function to plot correlation of different samples
quantilesOverPositions

show ChIP-chip data aligned over genome features, e.g. TSSs
cher-class

Class "cher" - ChIP-enriched region
autocor

Function to compute auto-correlation of probe intensities
nonzero-methods

Methods for Function nonzero
nimblegenScale

Function to compute scaled log-ratios
posToProbeAnno

Function for creating a probeAnno environment
extractProbeAnno

Build probeAnno from match positions in an RGList
compute.gc

Compute the GC content of DNA and probe sequences
plotBM

Visualization of a binary matrix
preprocess

Preprocess Raw ChIP-chip Intensities
validProbeAnno

Function to check a probeAnno environment
Ringo-internal

Internal Ringo functions
findChersOnSmoothed

Find ChIP-enriched regions on smoothed ExpressionSet
sliding.meansd

Compute mean and standard deviation of scores in a sliding window