Learn R Programming

⚠️There's a newer version (1.22.2) of this package.Take me there.

Rsubread (version 1.18.0)

Rsubread package: high-performance read alignment, quantification and mutation discovery

Description

This R package provides powerful and easy-to-use tools for analyzing next-gen sequencing read data. Functions of this package include quality assessment of sequence reads, read alignment, read summarization, exon-exon junction detection, fusion detection, detection of short and long indels, absolute expression calling and SNP calling. This package can be used to anlayze data generated from all major sequencing platforms such as Illumina GA/HiSeq/MiSeq, Roche GS-FLX, ABI SOLiD and LifeTech Ion PGM/Proton sequencers. It supports multiple operating systems incluidng Linux, Mac OS X, FreeBSD and Solaris.

Copy Link

Version

Version

1.18.0

License

GPL-3

Maintainer

Wei Shi

Last Published

February 15th, 2017

Functions in Rsubread (1.18.0)

align

Read mapping for genomic DNA-seq and RNA-seq data via seed-and-vote (Subread and Subjunc)
atgcContent

Calculate percentages of nucletodies A, T, G and C in a sequencing read datafile
detectionCallAnnotation

Generate annotation data used for calculating detection p values
createAnnotationFile

Create an annotation file from a GRanges object, suitable for featureCounts()
detectionCall

Determine detection p values for each gene in an RNA-seq dataset
featureCounts

featureCounts: a general-purpose read summarization function
exactSNP

exactSNP - an accurate and efficient SNP caller
findCommonVariants

Finding the common variants among all input VCF files
buildindex

Build index for a reference genome
getInBuiltAnnotation

Retrieve in-built annotations provided by featureCounts function
qualityScores

Extract quality score data in a sequencing read dataset
sam2bed

Convert a SAM format file to a BED format file
removeDupReads

Remove sequencing reads which are mapped to identical locations
RsubreadUsersGuide

View Rsubread Users Guide
processExons

Obtain chromosomal coordiates of each exon using NCBI annotation
propmapped

Calculate the proportion of mapped reads/fragments in SAM/BAM files