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SGSeq (version 1.4.0)

Splice event prediction and quantification from RNA-seq data

Description

Predict splice junctions and exons from BAM files and obtain compatible read counts and FPKMs. Identify splice events and estimate relative usage of splice variants based on compatible read counts at event boundaries.

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Version

Version

1.4.0

License

Artistic-2.0

Maintainer

Leonard Goldstein

Last Published

February 15th, 2017

Functions in SGSeq (1.4.0)

convertToTxFeatures

Convert to TxFeatures object
assays

Accessing and replacing assay data
exonCompatible

Compatible fragment counts for exons
exportFeatures

Export to BED format
filterFeatures

Filter predicted features
annotateSGVariants

Annotate splice variants in terms of canonical events
analyzeVariants

Analysis of splice variants
convertToSGFeatures

Convert transcript features to splice graph features
analyzeFeatures

Analysis of splice graph features from BAM files
annotate

Annotation with respect to transcript features
getSGFeatureCountsPerSample

Compatible fragment counts for splice graph features
getSGVariantCounts

Representative counts and frequency estimates for splice variants
findOverlapsRanges

Modified findOverlaps function for IRanges, IRangesList objects
getBamInfo

Obtain library information from BAM files
importTranscripts

Import transcripts from GFF file
findSGVariants

Find splice variants from splice graph
gr

Example genomic region of interest
getSGFeatureCounts

Compatible counts for splice graph features from BAM files
junctionCompatible

Compatible fragment counts for splice junctions
makeSGFeatureCounts

Create SGFeatureCounts object
makeVariantNames

Create interpretable splice variant names
mergeTxFeatures

Merge redundant features
predictCandidatesTerminal

Identify candidate terminal exons
predictCandidatesInternal

Identify candidate internal exons
plotSpliceGraph

Plot splice graph
plotVariants

Plot splice graph and heatmap of splice variant frequencies
predictExonsInternal

Identify internal exons
predictExonsTerminal

Identify terminal exons
plotCoverage

Plot read coverage and splice junction read counts
plotFeatures

Plot splice graph and heatmap of expression values
tx

Example transcripts
predictTxFeatures

Splice junction and exon prediction from BAM files
SGFeatureCounts

Constructor function for S4 class SGFeatureCounts
txf_ann

Example transcript features (annotation-based)
predictTxFeaturesPerSample

Identification of splice junctions and exons from BAM file
SGFeatures

Constructor function for S4 class SGFeatures
sgvc_pred

Example splice variant counts (predicted)
si

Example sample information
predictTxFeaturesPerStrand

Identification of splice junctions and exons for a given chromosome and strand
predictVariantEffects

Predict the effect of splice variants on protein-coding transcripts
sgvc_ann_from_bam

Example splice variant counts (annotated) from BAM files
SGVariants

Constructor function for S4 class SGVariants
sgf_pred

Example splice graph features (predicted)
sgfc_ann

Example splice graph feature counts (annotation-based)
sgf_ann

Example splice graph features (annotation-based)
sgfc_pred

Example splice graph feature counts (predicted)
slots

Accessing and replacing metadata columns
splicesiteOverlap

Compatible fragment counts for splice sites
processTerminalExons

Process predicted terminal exons
removeExonsIsolated

Remove exons with no flanking splice junctions
SGSegments

Constructor function for S4 class SGSegments
sgv_ann

Example splice variants (annotation-based)
TxFeatures

Constructor function for S4 class TxFeatures
predictJunctions

Identify splice junctions
sgv_pred

Example splice variants (predicted)
predictSpliced

IRanges-based identification of splice junctions and exons
txf_pred

Example transcript features (predicted)
SGVariantCounts

Constructor function for S4 class SGFeatureCounts
sgvc_ann

Example splice variant counts (annotated)
sgvc_pred_from_bam

Example splice variant counts (predicted) from BAM files