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SGSeq (version 1.4.0)

predictJunctions: Identify splice junctions

Description

Identify splice junctions from genomic RNA-seq read alignments.

Usage

predictJunctions(frag_exonic, frag_intron, min_junction_count, psi, retain_coverage)

Arguments

frag_exonic
IRangesList with exonic regions from alignments
frag_intron
IRangesList with introns implied by spliced alignments
min_junction_count
Minimum fragment count required for a splice junction to be included. If specified, argument alpha is ignored.
psi
Minimum splice frequency required for a splice junction to be included
retain_coverage
Logical indicating whether coverages for each exon should be retained as an IntegerList in metadata column “coverage”. This allows filtering of features using more stringent criteria after the initial prediction.

Value

IRanges of splice junctions with metadata columns “type” and “N”, and optionally “N_splicesite” for retain_coverage = TRUE