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beadarrayMSV (version 1.0.3)

beadarrayMSV-package: beadarrayMSV: Package for analysis of high-throughput Illumina BeadArrays

Description

Functions for reading, pre-processing and analyzing Illumina BeadArray data, in particular from the Infinium platforms. Methods for clustering and genotype calling of multisite variancs (MSVs) from polyploid genomes are provided. Paralogs may be resolved and mapped to individual chromosomes

Arguments

Details

ll{ Package: beadarrayMSV Type: Package Version: 1.0 Date: 2010-01-09 License: GPL (>=2) LazyLoad: yes LazyData: no Depends: R (>= 2.10.0), Biobase (>= 2.5.5), methods, geneplotter Imports: rggobi, limma SystemRequirements: GGobi } Two classes, "BeadSetIllumina" and "AlleleSetIllumina", are defined for holding and working with genetic data.

Illumina BeadArray summary-data are loaded using the function readBeadSummaryOutput, and pre-processed using preprocessBeadSet.

Automatic clustering and genotype calling is performed using callGenotypes, and validated using validateCallsPedigree and plotGenotypes.

Manual clustering with callGenotypes.interactive, which benefits from the interactive graphics package rggobi. Often used in combination with assignToAlleleSet.

Reading and writing data from and to files with the functions createAlleleSetFromFiles and writeAlleleSet.

For splitting MSV-5 markers into individual paralogs and assigning them to chromosomes, use assignParalogues.

References

L. Gidskehaug, M. Kent, B. J. Hayes, and S. Lien. (2010) Genotype calling and mapping of multisite variants using an Atlantic salmon iSelect SNP-array. Bioinformatics, doi: 10.1093/bioinformatics/btq673