Cluster raw data from QX100 and QX200 systems.
ddpcRquant(path, threshold.int = 0.9995, reps = 10, blocks = 150,
threshold.manual = NULL)
dpcr
object.
character
path to diectory with raw data.
numeric
probability of the threshold quantile.
numeric
vector representing the number of replications.
numeric
vector representing the number of blocks.
if numeric
, the value is used as the threshold. If
NULL
, the threshold is calculated automatically.
Wim Trypsteen, Matthijs Vynck, Jan De Neve, Pawel Bonczkowski, Maja Kiselinova, Eva Malatinkova, Karen Vervisch, Olivier Thas, Linos Vandekerckhove, Ward De Spiegelaere.
Trypsteen W, Vynck M, De Neve J, Bonczkowski P, Kiselinova M, Malatinkova E, Vervisch K, Thas O, Vandekerckhove L, De Spiegelaere W, ddpcRquant: Threshold Determination for Single Channel Droplet Digital PCR Experiments. Analytical and Bioanalytical Chemistry 2015. 407(19): p.5827-34.