BeadFlowFrame-class: BeadFlowFrame
Description
Extension of flowFrame specific for bead
data. The constructor take as arguments the FCS file and the
file containing the MEF values of the beads on the
different detector channels
Usage
BeadFlowFrame(fcs.filename, bead.filename)
Arguments
fcs.filename
The file name of the FCS to load.
File is loaded with the
read.FCS
function. bead.filename
The file name of the MEF
configuration files indicating the type of beads in the
FCS file. The bead.file is read with read.csv. Slots
fcs.filename:- The file name of
the FCS file from which to read.
bead.filename:- The file name of the bead
config file.
beads.mef:- The
data.frame containing the MEF of the bead
populations on different channels. trans:- The transform $f$ to linearise
the fluorescence.
inv.trans:- The inverse
transform of $f^{-1}$.