Description
An example FCS flowSet.
Format
This is an object of class flowSet composed of 7
flowFrames.Source
Data were generously provided by Clarient, Inc.Details
The flowSet corresponds to a Leukemia/Lymphoma panel for
a sample. Each flowFrame correponds to one tube. The
first one is an isotype control, and the rest are stained with
different antibody cocktails. Parameter 5 (FL3 Log) is notable
in that it is CD45-ECD for all of the tubes. This makes it possible
to gate the entire collection of tubes using CD45 vs SSC. This
set has a very similar distribution of CD45 vs SSC across all 7 tubes. column names:
FS Lin SS Log FL1 Log FL2 Log FL3 Log FL4 Log FL5 Log
References
W.T. Rogers and H. A. Holyst, “flowFP: A Bioconductor Package for Fingerprinting
Flow Cytometric Data”, Submitted to: Advances in Bioinformatics, April 1, 2009.