flowFP (version 1.30.0)

plate: 96 well plate data.

Description

An example of 96 well FCS flow data.

Usage

data(plate)

Format

This is an object of class flowSet comprising 96 flowFrames. Each flowFrame correponds to one sample. column names: FSC-H SSC-H FL1-H FL2-H FL3-H FL1-A FL4-H Time

Source

Original, non-sampled data are available at http://www.ficcs.org/software.html#Data_Files.

Details

This data set is derived from the references below. Each well in a 96-well plate is described by a flowFrame. Data were drastically down-sampled to 1000 events per flowFrame so that they could be included in this package. Note that flourescence parameters 5 (CD4 PerCP Cy5.5) and 7 (CD3 APC) are common across the entire set.

References

M. Inokuma, C. dela Rosa, C. Schmitt, P. Haaland, J. Siebert, D. Petry, M. Tang, M. A. Suni, S. A. Ghanekar, D. Gladding, J. F. Dunne, V. C. Maino, M. L. Disis, and H. T. Maecker. “Functional t cell responses to tumor antigens in breast cancer patients have a distinct phenotype and cytokine signature”. J Immunol, 179(4):2627-33, Aug 15 2007.

M. Inokuma, C. dela Rosa, C. Schmitt, P. Haaland, J. Siebert, D. Petry, M. Tang, M. A. Suni, S. A. Ghanekar, D. Gladding, J. F. Dunne, V. C. Maino, M. L. Disis, and H. T. Maecker, 2008. Data available at http://www.ficcs.org/software.html#Data_Files.

Examples

data(plate)