flowFit (version 1.10.0)

generationsDistance: Calculate the distance between 2 generations of cells on the FACS scale

Description

This function calculate the distance between 2 generations of cells on the FACS scale.

Usage

generationsDistance(dataRange, logDecades)

Arguments

dataRange

Digital Data range on the FACS instrument

logDecades

Number of log decades on the FACS instrument (dynamic range)

Value

Return the spacing between generations on the FACS scale.

Details

We can use this formula to convert FFI (FACS fluorescence Intensity) to RFI (Relative Fluorescence Intensity): $ RFI = 10^((FFI * l)/c) $

The inverse formula is used to convert from RFI to FACS fluorescence: $ FFI = (c*log(RFI)) / (l*log(10)) $

Where:

RFI

is the Relative Fluorescence Intensity

FFI

is the fluorescence on the FACS scale

$l$

is the number of log decades in the FACS instrument

$c$

is the number of data points (channels) in the instrument.

Using this formulas it is possible to estimate the spacing between generations on the FACS scale. The spacing value is automatically computed, based on the number of decades and the assumption that each generation has one-half of the intensity of the previous generation.

References

1. Tracking antigen-driven responses by flow cytometry: Monitoring proliferation by dye dilution. Paul K Wallace, Joseph D Tario, Jan L Fisher, Stephen S Wallace, Marc S Ernstoff, Katharine A Muirhead. Cytometry (2008) vol. 73A (11) pp. 1019-1034
2. FACS Formulas to convert between Relative Fluorescence and fluorescence on the FACS scale: MEASURING MOLECULES OF EQUIVALENT FLUOROCHROME (MEF) USING SPHEROTM RAINBOW AND ULTRA RAINBOW CALIBRATION PARTICLES, Spherotech, http://www.spherotech.com/tech_SpheroTech_Note_9.html

Examples

  distance <- generationsDistance(1024, 4)