setup

A dataset containing the description of an experimental setup where
96 larvae of Galleria mellonella have been injected with a culture
of the bacterium Xenorhabdus nematophila.

Use spectrophotometry measurements performed on insects as a way to infer pathogens
virulence. Insect movements cause fluctuations in fluorescence signal, and functions are
provided to estimate when the insect has died as the moment when variance in autofluorescence
signal drops to zero. The package provides functions to obtain this estimate together with
functions to import spectrophotometry data from a Biotek microplate reader. Details of the method
are given in Parthuisot et al. (2018) <doi:10.1101/297929>.

Jean-Baptiste Ferdy

fluoSurv

Estimate Insect Survival from Fluorescence Data

setup function

A data frame with 96 rows and 4 variables:<dl class="dl-horizontal">
 <dt>well</dt><dd>Well name.</dd>
 <dt>dilution</dt><dd>Dilution factor (log-transformed) of the injected culture. 1 therefore means 10 fold dilution, while LB corresponds to negative control where insects have been injected with sterile LB culture medium.</dd>
 <dt>time_injection</dt><dd>Time of the injection.</dd>
 <dt>dead</dt><dd>Is the insect dead at the end of the experiment?</dd>
</dl>

Format

An injection experimental setup — setup

A data frame with 96 rows and 4 variables:<dl class='dl-horizontal'>
 <dt>well</dt><dd>Well name.</dd>
 <dt>dilution</dt><dd>Dilution factor (log-transformed) of the injected culture. 1 therefore means 10 fold dilution, while LB corresponds to negative control where insects have been injected with sterile LB culture medium.</dd>
 <dt>time_injection</dt><dd>Time of the injection.</dd>
 <dt>dead</dt><dd>Is the insect dead at the end of the experiment?</dd>
</dl>

setup: An injection experimental setup

Description

Arguments

Format