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girafe (version 1.24.0)

Genome Intervals and Read Alignments for Functional Exploration

Description

The package 'girafe' deals with the genome-level representation of aligned reads from next-generation sequencing data. It contains an object class for enabling a detailed description of genome intervals with aligned reads and functions for comparing, visualising, exporting and working with such intervals and the aligned reads. As such, the package interacts with and provides a link between the packages ShortRead, IRanges and genomeIntervals.

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Version

Version

1.24.0

License

Artistic-2.0

Maintainer

J Toedling

Last Published

February 15th, 2017

Functions in girafe (1.24.0)

perWindow

Investigate aligned reads in genome intervals with sliding windows
which_nearest-methods

Methods for function 'which_nearest' and genome intervals
Additional reduce-methods

Auxiliary methods for Function reduce in Package 'girafe'
intPhred

Extract integer Phred score values from FastQ data
weightedConsensusMatrix

compute weighted consensus matrix
medianByPosition

Compute median quality for each nucleotide position
plotReads

Function to plot aligned reads along the chromosome
AlignedGenomeIntervals-class

Class 'AlignedGenomeIntervals'
plotAligned

Visualise reads aligned to genome intervals
countReadsAnnotated

Sum up aligned reads per category of genome feature
getFeatureCounts

get the read counts for a supplied set of genomic features
plotNegBinomFit

Plot Negative Binomial Fit
agiFromBam

Create AlignedGenomeIntervals objects from BAM files.
fracOverlap

Retrieve intervals overlapping by fraction of width
girafe-internal

Internal girafe functions
trimAdapter

Remove 3' adapter contamination
addNBSignificance

assess significance of sliding-window read counts