normfluodbf (version 1.5.2)

fluor_threshold_check: Title: A fluorescence quantification Quality Control (QC) function.

Description

A function designed to check that fluorescence values do not exceed the upper limit (2^15 or 32768) OR fall below the lower limit (2^11 or 2048). Fluorescence values that exceed these thresholds are considered noisy and lead to incorrect interpretation of analysis results.

Usage

fluor_threshold_check(clean_df, fun = NA)

Value

A polite warning message to the data analyst or researcher.

Arguments

clean_df

A cleaned data frame.

fun

A parameter used for Boolean expressions.

Author

Tingwei Adeck

See Also

fluor_threshold_check_na(), fluor_threshold_check_raw()

Examples

fpath <- system.file("extdata", "dat_1.dat", package = "normfluodbf", mustWork = TRUE)
dat_df <- read.table(file=fpath)
nocomma_dat <- clean_odddat_optimus(dat_df)
resampled_scaled <- resample_dat_scale(nocomma_dat, tnp=3, cycles=40)
resampled_scaled <- resampled_scaled[,c(1:4)]
fluor_threshold_check(resampled_scaled)