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plateCore (version 1.30.0)

pbmcPlate: pbmcPlate Data Set

Description

One 96-well plate from a BD FACS CAP analysis of Peripheral Blood Mononucleocyte (PMBC) cells.

Usage

data(plateCore)

Arguments

Format

The format is an object of class flowSet composed of 96 flowFrames. Each flowFrame corresponds to one well from the plate.

Source

Complete dataset available at http://www.ficcs.org/software.html#Data_Files, the Flow Informatics and Computational Cytometry Society website (FICCS)

Details

BD FACS CAP (http://www.bd.com/technologies/discovery_platform/BD_FACS_CAP.asp) is a platform for screening a large number of antibodies (200+) on human samples. Antibodies are arrayed 3-per well on a 96-well plate, along with the appropriate controls. In this experiment, an early version of FACS CAP was used to screen PBMCs from 2 donors for 189 different human cell surface markers. The complete data set is available from the FICCS site shown below. The pbmcPlate include with plateCore is a lymphocyte enriched subset of one of the replicate plates for donor 1.

References

Add reference for plateCore paper (when/if published).