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qpcR (version 1.3-3)

Modelling and analysis of real-time PCR data

Description

Model fitting, optimal model selection and calculation of various features that are essential in the analysis of quantitative real-time polymerase chain reaction (qPCR).

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Version

Install

install.packages('qpcR')

Monthly Downloads

1,736

Version

1.3-3

License

GPL (>= 2)

Maintainer

Andrej-Nikolai Spiess

Last Published

November 3rd, 2010

Functions in qpcR (1.3-3)

S27

qPCR data of the S27a transcript in 15 testicular biopsies
akaike.weights

Calculation of Akaike weights/relative likelihoods/delta-AICs
efficiency

Calculation of qPCR efficiency and other important qPCR parameters
Rsq

R-square value of a fitted model
reps3

qPCR dilution experiments Set #3 from the package author
calib

Calculation of qPCR efficiency by dilution curve analysis
reps2

qPCR dilution experiments Set #2 from the package author
resVar

Residual variance of a fitted model
pcropt1

Combinatorial elimination of plateau and ground phase cycles
modlist

Create nonlinear models from a dataframe and coerce them into a list
propagate

General error analysis function using different methods
dyemelt

Melting curves of a 4-plex qPCR with different fluorescence dyes
guescini2

qPCR dilution experiments Set #2 from Guescini et al. (2008)
RSS

Residual sum-of-squares of a fitted model
expfit

Calculation of PCR efficiency by fitting an exponential model
mselect

Sigmoidal model selection by different criteria
eff

The amplification efficiency curve of a fitted object
sliwin

Calculation of PCR efficiency by the window-of-linearity method
guescini1

qPCR dilution experiments Set #1 from Guescini et al. (2008)
pcrimport

Simple qPCR data import function (i.e. from text files or clipboard)
RMSE

Root-mean-squared-error of a fitted model
evidence

Evidence ratio for model comparison with AIC or AICc
expcomp

Comparison of all sigmodal models within the exponential region
ratiocalc

Calculation of ratios from qPCR runs with/without reference data
curvemean

Building a model which averages a batch of qPCR curves
resplot

A residuals bar-plot with colour-coded bars
PRESS

Allen's PRESS (Prediction Sum-Of-Squares) statistic, aka P-square
plot.pcrfit

Plotting qPCR data with fitted curves/confidence bands/error bars
rutledge

qPCR dilution experiments from Rutledge et al. (2004)
pcrfit

Function for qPCR model fitting
pcrbatch

Batch calculation of qPCR efficiency and other qPCR parameters
reps

qPCR dilution experiments Set #1 from the package author
AICc

Akaike's second-order corrected Information Criterion
pcrGOF

Summarize measures for the goodness-of-fit