qpcR (version 1.3-3)

dyemelt: Melting curves of a 4-plex qPCR with different fluorescence dyes

Description

A melting curve analysis of a multiplex real-time PCR on genomic DNA, producing 4 amplicons. The fluorescence dyes used were EvaGreen, SybrGreen I and Syto-13.

Usage

data(dyemelt)

encoding

latin1

format

A data frame with the temperature values in columns 1, 2, 3 and the corresponding fluorescence data in columns 2, 4, 6.

Details

The melting curve was conducted with AZF deletion-specific primers in a Lightcycler 1.0 instrument (Roche).

Examples

data(dyemelt)
plot(dyemelt[, 1], dyemelt[, 2], xlab = "Temperature [�C]", 
     ylab = "Raw fluorescence")