spotSegTest: Spot Segmentation Test Data
Description
The two columns of this data set represent the Cy3 (green) absorption
intensities for channel 1,
and the Cy5 (red) absorption intensities for channel 2
for part of a dye-swap experiment with replicates.
They measure expression levels of
cellular RNA transcripts assessed in CD4+ T cell lines at different
times after infection with HIV-1BRU using DNA microarrays.
Format
Each column is a vector of intensities of 24 spots arranged in
4 rows and 6 columns, encoded for compact (16-bit TIFF) storage.
For processing each column of spotSegTest
should first be converted to a 144x199
matrix, then applying the transformation described below.Details
The intensities can be obtained from this data by first
subtracting them from 65535 (256*256-1), then squaring, then multiplying by
a scale factor 4.71542407E-05.
In other words, a number x in the spotSegTest data set
corresponds to intensity (256*256 - 1 - x)^2*.0000471542407
. \
References
van't Wout AB, Lehrman GK, Mikheeva SA, O'Keeffe GC, Katze MG, Bumgarner RE,
Geiss GK, Mullins JI\
Cellular gene expression upon human immunodeficiency virus type 1 infection of CD4(+)-T-cell lines.\
J Virol. 2003 Jan;77(2):1392-402.