piRNA ping-pong analysis of complementary sequences
Usage
pingpong(bam_file)
Arguments
bam_file
An object of class data.frame or DataFrame
Value
This function returns a data.frame object with frequency of overlapping complementary piRNAs.
Details
The ping-pong mechanism is a proposed method for the amplification of primary piRNAs, which leads to the production of new primary piRNAs from their precursor transcripts, which eventually amplifies the pool of both primary and secondary piRNAs. This positive feedback loop is a secondary biogenesis mechanism that requires complementary transcripts to a pre-existing pool of piRNAs.
References
Brennecke J. et al. Cell 128, 1089-1103, March 23, 2007