High-Throughput SPR Kinetics Analysis Software

This R package provides functions and a pipeline to analyze SPR data. It takes as input the sample information and the Carterra time series and produces a pdf with sensorgrams, fits, rate constants, Rmax, plots of residuals and standard errors of parameter estimates. It also produces a .csv file with the relevant parameters and standard errors.

Current features include:

• 1:1 binding model
• Options for local or global Rmax fitting
• Automated concentration range determination
• Automated dissociation window approximation
• Automated bulkshift determination

The motivations for developing this package are

• to provide an open-source tool for analyzing high-throughput SPR kinetic data
• to automate some of the steps in analysis, such as determining the length of dissociation window and concentration range to include in fits and whether or not to include bulk-shift in fitting.
• to provide a platform for further development of multivalent binding kinetics models, such as bivalent analyte, parallel binding and more. These models are currently not an option in commercial software when using non-regenerative SPR data.